|
KMID : 1140120090140040350
|
|
Cancer Prevention Research
2009 Volume.14 No. 4 p.350 ~ p.358
|
|
|
Apoptosis Induction by Aged Black Garlic Extracts in AGS Human Gastric Carcinoma Cells
|
|
Shin Dong-Yeok
Yoon Moo-Kyoung
Choi Young-Whan
Gweon Oh-Cheon
Kim Jung-In
Choi Yung-Hyun
|
|
|
Abstract
|
|
|
|
Garlic is generally used as a therapeutic reagent against various diseases, and many studies have indicated that garlic and its derivatives can reduce the risk of various types of human cancer. In this study, the potential of water extract of raw garlic (RG) and water extract of aged black garlic (ABG) to induce apoptosis in human gastric carcinoma AGS cells was investigated. Treatment of AGS cells to ABG, but not RG, resulted in the inhibition of cell growth and viability and the induction of apoptosis, which could be proved by MTT assay, DAPI staining and flow cytometry analysis. The increase in apoptosis that was induced by ABG was correlated with down-regulation of anti-apoptotic Bcl-2 and Bcl-xL expression, up-regulation of pro-apoptotic Bax, Fas and Fas ligand, and a decrease in the mitochondrial membrane potential. ABG treatment induced the proteolytic activation of caspases and a concomitant degradation of poly (ADP-ribose)-polymerase. Cleavage of Bid and a down-regulation of the inhibitor of apoptosis family proteins were also observed in ABG-treated AGS cells. Taken together, the data from this study indicates that ABG induces the apoptosis of AGS cells through a signaling cascade of death receptor-mediated extrinsic and mitochondria-mediated intrinsic caspase pathways.
|
|
|
KEYWORD
|
|
|
Aged black garlic, AGS, Apoptosis, Caspases
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|
|